- Hemostasis and thrombosis
- Medical University of Vienna
Thrombospondin-1 (TSP-1) is a protein which is secreted by activated platelets and endothelial cells and promotes blood clotting by various mechanisms: TSP-1 supports platelet adhesion, it functions as a bridging molecule in platelet aggregation, and it protects platelet strings from degradation. Inflammatory cells such as neutrophilic granulocytes can process thrombospondin-1 to a smaller protein variant which has more potent hemostatic properties.
This Master´s project is designed to characterize the cellular interactions and molecule turnover of these TSP-1 isoforms in more detail: Fusion variants of TSP-1 and fluorescent proteins will be constructed, recombinantly produced and purified. Platelets deficient in TSP-1 will be isolated from TSP-1 k.o. mice and exposed to co-culture with neutrophils. The cell binding, internalization and degradation of fluorescent TSP-1 fusion proteins will be monitored by confocal laser scanning microscopy.
This project is conducted in the context of an FWF-funded research consortium (FWF SFB-54; https://inthro.meduniwien.ac.at/) which addresses the link between inflammation and thrombosis. It is designed to determine whether proteolytic processing of TSP-1 is a mechanism by which inflammatory reactions can alter the function of TSP-1 thereby promoting blood clotting and the tendency for thrombotic events.
We are offering a position for a Master´s student in a full-time engagement for 1 year, including a monthly scholarship of € 440,-
Applicants with a Bachelor's degree in molecular biology, genetics or a related discipline should submit their application (letter of interest, CV) by email to
Christine Brostjan, Ph.D.
Associate Professor for Vascular Biology
Department of Surgery
Medical University of Vienna
Anna Spiegel Center for Translational Research
General Hospital AKH 25.05.002
Waehringer Guertel 18-20
A - 1090 Vienna, Austria
Phone: +43 1 40400 73514
- Name: Christine Brostjan
- Telefon: +43 1 40400 73514
- Fax: +43 1 40400 73593