WANTED: PhD student
- TU Wien
- Closing Date
Recombinant horseradish peroxidase for targeted cancer treatment
The goal of this FWF project (P30872-B26) is to engineer the enzyme horseradish peroxidase (HRP) towards higher stability and activity with the plant hormone indole-3-acetic acid (IAA) and paracetamol for targeted cancer treatment. For that purpose, HRP and HRP variants will be expressed in E. coli in an unglycosylated form. After screening of HRP variants, the mutated HRP lead candidate will be produced as Inclusion Bodies in the controlled environment of a bioreactor in the gram scale, processed, purified, biochemically characterized, conjugated to antibodies and lectins and tested in vitro in combination with IAA and paracetamol on various cancer cell lines to demonstrate its potential use for targeted cancer treatment.
You will be able to do mutational studies with the enzyme (rational protein design and directed evolution), conduct all experiments in the Upstream and the Downstream (including preparation, execution and process control of batch and fed-batch cultivations, product analysis of cultures, Inclusion Body processing, product purification, product analyses as well as careful evaluation of the obtained large data sets), and do conjugation experiments as well as in vitro experiments with cancer cell lines abroad.
You are studying biotechnology, biochemistry, pharmacy, bioprocess technology or similar. You have experience in molecular biology, fermentation technology, an affinity for data evaluation and ideally experience with Downstream techniques. Moreover, you are able to work independently as well as in a team. The ability to meet time lines, good written and verbal communication skills and a safe command of English are also part of your qualifications.
The position is available from January 2018 and is scheduled for 3.5 years.
- Main contact
- Name: Oliver Spadiut
- Phone: +43 1 58801 166473