Master Student Position in RNA Biology @ Center for Anatomy and Cell Biology
- RNA Biology
- Medical University of Vienna
- Closing Date
Adenosine to inosine editing is a co-transcriptional process that causes deamination of adenosine and conversion into inosine. Inosine is read as guanosine by cellular machines. This can lead to recoding of transcripts, consequences for the innate immune response, and differences in miRNA-maturation and targeting (1). A tight regulation and control of RNA-editing is required for homeostasis as de-regulation of editing has been linked to various diseases including neurological disorders or cancer. We have found surprising differences in the level of pre-mRNA and mRNA editing for a number of editing targets (2).
This finding may provide a novel layer of regulation for editing (3). Therefore, we plan to use NGS (amplicon-seq) in order to screen the whole protein-coding editome for similar differences. Moreover, we will use a combination of affinity-purifications and mass spectrometry in order to search for regulatory proteins that can explain the differences in the level of editing. Finally, we plan to validate candidate proteins using CRISPR knockout cell lines or lentiviral knockdowns.
To address these questions we offer an exciting Master Thesis project entitled:
“Controlling the A-to-I editing landscape”
The applicant requires a background in the field of molecular biology, biomedical sciences, or biochemistry.
Starting Date: 04/2018. The position will be paid 440 EUR/month.
Please send your CV and letter of motivation to: konstantin.licht(at)meduniwien.ac.at
1) Rapid and dynamic transcriptome regulation by RNA editing and RNA modifications. Licht K, Jantsch MF. J Cell Biol. 2016 Apr 11;213(1):15-22.
2) Adenosine to Inosine editing frequency controlled by splicing efficiency. Licht K, Kapoor U, Mayrhofer E, Jantsch MF. Nucleic Acids Res. 2016 Jul 27;44(13):6398-408.
3) Dynamic landscape and regulation of RNA editing in mammals. Tan MH et al. Nature. 2017 Oct 11;550(7675):249-254.
- Main contact
- Name: Konstantin Licht