Description of project:

Enzyme interactions with polymer substrates at the nexus of the soluble and insoluble state are critical in the biocatalysis of polymer synthesis and degradation. Understanding of these interactions is still limited which complicates engineering of enzymes for activity on polymers. In this project we study the polymer interactions of enzymes involved in the degradation of lignocellulosic materials. We also study the degradation of plastics. In addition, we study controlled synthesis of polysaccharide materials using enzymatic polymerization.

Research Objectives:

• Preparation of polymer substrates suitable for single enzyme molecule analysis

• Biophysical characterization of enzyme interactions with different polymer substrates

• Bottom-up enzymatic synthesis of polysaccharide materials with tunable properties

• Characterization of synthetic materials

Methods:

In situ atomic force microscopy, surface plasmon resonance, enzymatic reaction, product characterization in solution (e.g., HPLC, NMR) and in solid state

Embedding in the cluster Circular Bioengineering:

COE program 1, researcher groups working with enzymes and polymers (e.g., Ribitsch, Kracher), connection to COE program 3 in respect to enzymatic synthesis.

Application guidelines:

Please send your job application to cb_recruiting(at)boku.ac.at, indicating your name and the project/position number in the subject line (e.g. "Jane Doe, application for PD 1.7").

Further information on Application documents and formal criteria see https://www.circularbioengineering.at/ Application guidelines - Postdocs — circular bioengineering. The application must meet all formal criteria, otherwise it cannot be considered.

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