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Job-Art: Wissenschaftliche Jobausschreibungen

Master thesis

Fachgebiet
Cancer Research
Arbeitsstätte
Ludwig Boltzmann Institute for Cancer Research
Website
Bewerbungsfrist
31.05.2017

Ausschreibungstext

Blood cancer metabolism control through the STAT5 transcription factor and JAK2 kinase

The JAK2-STAT5 core cancer signaling pathway gained increased attention due to frequent hyperactivation. STAT5 activation and tyrosine phosphorylation is mainly triggered by JAK tyrosine kinases, themselves frequently mutated and hyper-activated in cancer. We published in 2017 (Freund et al., Leukemia, 2017) on a new post-translational modification, namely O-GlcNAc glycosylation of STAT5 at threonine 92 to be essential for transformation identifying a metabolic sensor linking cancer cell growth to metabolism (http://lbicr.lbg.ac.at/en/metabolism-drives-growth-and-division-cancer-cells). We used a hyperactive gain-of-function variant of STAT5 lacking O-GlcNAc and now we address the upstream JAK2 kinase for similar modification. We have established tools for STAT5 and for JAK2 to study the changes in O-GlcNAcylation under variable nutritional status being either high or low in glucose or glutamine. Within the MSc thesis we want to analyze O-GlcNAc-modified JAK2 in human cancer cells and map its O-GlcNAc modification by mutagenic analysis. We will generate an O-GlcNAc-deficient hyperactive JAK2V617F variant. Furthermore, we will reconstitute STAT5- or JAK2-deficient mouse embryonic fibroblasts with specific STAT5- or JAK2-O-GlcNAc-deficient variants to gain insight into transcriptional target gene regulation using gene expression profiling with RNA-Seq and bioinformatics analysis. Moreover, ChIP-Seq is planned for essential histone modifications and STAT5.

Freund et al. O-GlcNAcylation of STAT5 controls tyrosine phosphorylation and oncogenic transcription in STAT5-dependent malignancies. Leukemia; doi: 10.1038/leu.2017.4

We are looking for a talented and highly motivated MSc student with interest in cancer research with the following skills.

  • Knowledge in biology
  • Accuracy and reliability
  • Interpersonal skills and ability to work in an international team
  • Written and oral communication skills in English
  •  Experience with cell culture, biochemical and molecular biology methods

The MSc student will gain experience in cell culture techniques, FACS, cloning, transfection, PCR, qPCR, Western blotting, IP, RNA-Seq, bioinformatics analysis basics and ChIP-Seq protocols. Participation in group meetings and progress reports of the institute will encourage scientific communication skills.

Application Details:

Duration: 10-12months incl. written Master thesis with the possibility of extension

Compensation: 440 €/month

Place of work: Veterinärplatz 1; 1210 Vienna; Austria

Start: as soon as possible

Application: Please send per email your cover letter, detailed Curriculum Vitae and arrange for at least one letter of recommendation

Kontakt-Person(en)

Haupt-Kontakt
Name: Patricia Freund

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