Master Student Position
- Molecular Cell Biology, Stem Cell Differentiation, Cancer Research
- Währingerstrasse 10, 1090 Vienna
The research team of Assoc. Prof. Mario Mikula is seeking a talented and motivated MSc student to work on cellular mechanisms contributing to melanoma progression. Title of Master Thesis:"The EMT phenoytpe in melanoma, mechanisms leading to HIF and TGFbeta activation."
Metastatic tumors are hallmarked by a pronounced EMT phenotype. We are interested in mechanisms necessary to establish and maintain this process in melanoma (see our related publications). We seek for a motivated Master student to strengthen our efforts in elucidating regulators of HIF and TGF-beta signalling in 2D, 3D and in vivo models of melanoma. Tasks will involve the establishment of stable overexpressing cell lines. Additional tasks include: immunohistochemical or confocal staining of tumor tissue and quantification of staining via electronic methods. A major task will be the application of methods to evaluate tumor suppressor function of overexpressed proteins as well measuring tumor migration and invasion.
We seek for a MSc student with the following skills:
• Applicants should hold a BSc degree and have completed their undergraduate studies
• Accuracy and reliability
• Written and oral communication skills in English
We offer a stimulating work environment with many possibities to expand his or her knowledge in the field of molecular cell and tumor biology. We will provide training for bioinformatic analysis as well as an introduction to analysis of clinical patient samples. The selected study will participate in group meetings and progress reports at our institute.
References of recent work relevent for this position:
1. Linke M, Pham HT, Katholnig K, Schnoller T, Miller A, Demel F, et al. Chronic signaling via the metabolic checkpoint kinase mTORC1 induces macrophage granuloma formation and marks sarcoidosis progression. Nat Immunol 2017;18(3):293-302.
2. Schutz B, Koppensteiner A, Schorghofer D, Kinslechner K, Timelthaler G, Eferl R, et al. Generation of metastatic melanoma specific antibodies by affinity purification. Sci Rep 2016;6:37253.
3. Preitschopf A, Schorghofer D, Kinslechner K, Schutz B, Zwickl H, Rosner M, et al. Rapamycin-Induced Hypoxia Inducible Factor 2A Is Essential for Chondrogenic Differentiation of Amniotic Fluid Stem Cells. Stem Cells Transl Med 2016;5(5):580-90.
4. Schorghofer D, Kinslechner K, Preitschopf A, Schutz B, Rohrl C, Hengstschlager M, et al. The HDL receptor SR-BI is associated with human prostate cancer progression and plays a possible role in establishing androgen independence. Reprod Biol Endocrinol 2015;13:88.
5. Preitschopf A, Li K, Schorghofer D, Kinslechner K, Schutz B, Thi Thanh Pham H, et al. mTORC1 is essential for early steps during Schwann cell differentiation of amniotic fluid stem cells and regulates lipogenic gene expression. PLoS One 2014;9(9):e107004.
6. Swoboda A, Schanab O, Tauber S, Bilban M, Berger W, Petzelbauer P, et al. MET expression in melanoma correlates with a lymphangiogenic phenotype. Hum Mol Genet 2012;21(15):3387-96.
Duration: 10-12months incl. written Master thesis with the possibility of extension
Compensation: 440 €/month
Start: as soon as possible
Application: Please send per email your application letter, detailed Curriculum Vitae and arrange for at least one letter of recommendation. It is mandatory to attach a protocol from a practical training of your choice, where you 1) collected data, 2) analysed data and 3) interpreted data.
- Name: Mario Mikula