Making (cytosine-5) RNA methylation programmable using RNA-targeting Cas9 (RCas9)
- Basics in molecular biology and tissue culture work
- Center for Anatomy and Cell Biology, Division of Cell and Developmental Biology
The project is based on a recently developed approach using nuclease-inactive S. pyogenes CRISPR/Cas9 that can bind RNA in a nucleic-acid-programmed manner allowing to manipulate endogenous RNAs in living cells.
The focus of this project will be developing a system that allows introducing m5C at specific positions (5’-UTR, 3’-UTR, splice junctions, coding sequence) of a reporter RNA in mammalian cell culture and testing the effects of single m5C sites on RNA metabolism and translation. For more information, visit our website or email: matthias.schaefer(at)meduniwien.ac.at
- Name: Matthias Schaefer