PhD position in Oligonucleotide Synthesis

Fachgebiet

Nucleic Acid Chemistry/Bioorganic Chemistry

Arbeitsstätte

University of Vienna, Faculty of Chemistry

Website

https://anorg-chemie.univie.ac.at/research/nucleic-acid-chemistry/

Bewerbungsfrist

31.01.2021

Ausschreibungstext

The research group „Nucleic Acid Chemistry” at the Institute of Inorganic Chemistry of the University of Vienna is looking for a PhD candidate

to work on an organic/bioorganic chemistry project involving a novel approach for the chemical synthesis of RNA oligonucleotides at very high throughput using a photolithographic array fabrication process. The position is to be filled for a period of three years, starting right away.

ROLE WITHIN THE RESEARCH PROJECT

• Incorporate new photosensitive RNA phosphoramidites into oligonucleotides synthesized in situ by microarray photolithography

• Synthesize long RNA oligonucleotides at very high density

• Optimize synthetic parameters to reach RNA synthesis throughputs comparable to DNA synthesis

• Generate sequence permutations to study the binding properties of fluorogenic aptamers

• Participate in DNA and RNA library synthesis for gene assembly, sequencing and data storage applications

• Expand high-density array synthesis to chemically-modified nucleoside analogs

• Coordinate with collaboration partners

• Writing scientific articles and participation to symposiums/conferences

PROFILE

• MSc degree in bioorganic/organic chemistry (strongly preferred), biochemistry, synthetic/molecular biology

• Experience in the handling, preparation and analysis of nucleic acids is a strong asset (solid-phase synthesis, phosphoramidite chemistry, PCR, library preparation…)

• General understanding of sequencing methods, bioinformatics and programming is a plus

• Excellent command of written and spoken English. French and German are a plus

• Excellent teamwork and communication skills, independent, critical thinking and initiative

WORKING ENVIRONMENT AND CONDITIONS

• 30 h/week, salary corresponding to doctorant-level according to the FWF personnel costs (~€31000 brutto/year)

• A stimulating research environment, dedicated to problem-solving and opened to new ideas and hypotheses

• Access to the benefits and facilities of the University of Vienna, the largest research institution in Austria

ADDITIONAL INFORMATION

• https://anorg-chemie.univie.ac.at/research/nucleic-acid-chemistry/

To apply, please send CV and motivation letter, as well as at least one letter of recommendation to Dr. Jory Lietard (jory.lietard(at)univie.ac.at) no later than 31/01/21.

Kontakt-Person(en)

Haupt-Kontakt

Name: Jory Lietard

E-Mail: jory.lietard(at)univie.ac.at

Telefon: 00431427752643