Master Thesis "Glycosyltransferases for Plant Cell Wall Glycoproteins
- plant biochemistry, glycobiology
- University of Natural Resources and Life Sciences, Vienna
Arabinogalactan proteins (AGPs) are a major class of glycoproteins in the plant cell wall and are involved in many developmental processes. These proteins are anchored to the plasma membrane and are decorated with complex and heterogeneous O-linked type II arabinogalactan (AG) polysaccharides. Despite their importance, the function of the AG polysaccharide is largely unknown and only few glycosyltransferases involved in its biosynthesis have been identified. In your Master Thesis, you will perform a biochemical screen to identify novel plant glycosyltransferases involved in glycosylation of arabinogalactan proteins. The methodologies include enzyme expression using a mammalian cell culture system, molecular cloning of glycosyltransferase genes, and enzyme assays for analyzing the substrate specificities of the glycosyltransferases.
Start: September 2022 or as soon as possible thereafter
Duration: 6 months
Payment: „geringfügig“, i.e. ~470€/month
- Name: Colin Ruprecht