- Cancer Research
- Ludwig Boltzmann Institute for Cancer Research
- Closing Date
The research team of Richard Moriggl is seeking a talented and motivated Master-student to join the laboratory the Ludwig Boltzmann Institute for Cancer Research, Veterinary University of Vienna to work on an ambitious research project:
The molecular mechanism of STAT5 sensing metabolic status
STAT5 proteins are key transcription factors gaining increased attention as essential drivers of cancer. Recently, recurrent STAT5 mutations causing hyper-activated gene transcription were identified from cancer genome sequencing efforts. STAT5 activation and tyrosine phosphorylation is mainly triggered by JAK tyrosine kinases, themselves frequently mutated and hyper-activated in cancer. An additional post-translational modification, the O-linked β-DN-acetylglucosamine (O-GlcNAc) glycosylation of STAT5 at threonine 92 is essential for transformation. We used a hyperactive gain-of-function variant of STAT5 lacking O-GlcNAc at threonine 92 to study the impact of O-GlcNAc modification of STAT5 in a recent publication.
We aim to generate deeper mechanistic insight into the role of O-GlcNAcylation of STAT5 to study the role of JAK2 in hematopoietic cancer. The tools, which we have established for STAT5 will be further expanded to JAK2 to study the changes in O-GlcNAcylation under variable in nutritional status. Furthermore, we will also reconstitute STAT5-deficient mouse embryonic fibroblasts with multiple STAT5 O-GlcNAc variants to gain insight into transcriptional target gene regulation using techniques such as RNA-Seq, ATAC-Seq, DNA methylation, ChIP-Seq and bioinformatics data evaluations.
The Master’s Candidate Profile:
We are looking for a talented and highly motivated master student with interest to perform basic and translational cancer research. The student will gain experience in cell culture techniques, flow cytometry, cloning, transfection, PCR, qPCR, Western blotting, IP, NGS, and bioinformatics. Furthermore, participation in group meetings and progress reports of the institute will encourage the student’s scientific communication skills.
Advanced knowledge in biology
High accuracy and reliability
Good interpersonal skills and ability to work in an international team
Good written and oral communication skills in English
Previous experience with cell culture, biochemical and molecular biological methods is highly desirable
Practical laboratory experience prior to start is mandatory
Duration: 12 months incl. written Master thesis with the possibility of extension
Compensation: EUR 440,- netto/month
Place of work: Veterinary University Vienna
Start: as soon as possible
Application: Please send per email your cover letter, detailed curriculum vitae and arrange for at least one letter of recommendation of a supervisor of your previous practical lab experience by April 1, 2017.
- Main contact
- Name: Patricia Freund