Description of project:

Enzymes will be engineered by fusion with metal-binding peptides or protein binding modules to immobilize them on electrode materials (e.g. platinum, gold, carbon). For this purpose, metal-binding peptides are screened by phage display and naturally occurring protein binding modules are engineered. Candidate peptides and proteins will be tagged to the bioelectrocatalysts for oriented immobilization and the reactions electrochemically investigated.

Background:

Enzymes as bioelectrocatalysts need to be connected to an electrode. Chemical immobilization methods lead to random orientations of the enzyme on the electrode and deactivation. The oriented immobilization of enzymes in a monolayer can be achieved by peptide or protein sequences/modules that bring the enzymes in productive contact with the electrode.

Research Objectives:

• In silico and in vitro screening of peptides and protein binding to electrode materials

• Protein modelling and engineering for fusion proteins, production of fusion proteins

• Biochemical characterization and electrochemical testing

Methods:

• Affinity screening (fluorescence microscopy, SPR)

• Phage display screening of metal-binding peptides using model metal surfaces

• Protein engineering (Rational design) of protein binding modules

• Recombinant protein expression and purification

• Biochemical and electrochemical characterization

Where to apply:

https://www.circularbioengineering.at/

Fachgebiet

Biochemistry, Electrochemistry, Protein Engineering, Materials Science, Analytical Chemistry

Arbeitsstätte

BOKU University

Stunden

30

Antrittsdatum

01. September 2025

Bewerbungsfrist

30. Juni 2025

Kontakt

Cluster of Excellence: Circular Bioengineering
cb_managingdirector(at)boku.ac.at
014765475002

Sophie Schober
cb_managingdirector(at)boku.ac.at
014765475002

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