Description of project:

Development of efficient bio-electrocatalytic systems based on advanced understanding, and engineering, of the functional interactions between enzymes and electrode surfaces; design of enzymes and surfaces for optimized interaction for mediated or direct electron transfer with electrodes

Background:

Bio-electrocatalysis is promising to perform chemical transformations with minimal use of reagents and low waste produced. The design of electrochemical reactors often requires that enzyme catalysts are immobilized on the electroactive surfaces of electrodes. Electrode materials (e.g., metals, carbon) are often poorly suitable to provide an interface for interaction with the enzyme catalysts for immobilization. The project aims to deepen the understanding of enzyme interactions with electrode materials in order to enable more efficient engineering of functional bio-interfaces in, and for, electrochemical transformations.

Research Objectives:

- Exploring and optimizing enzyme interactions with carbon-based electron surfaces

- Designing enzymes for improved active adsorption on electrodes

- Engineering of enzyme-surface interactions for direct electron transfer

- Characterization of enzyme electrodes for mediated and direct electron transfer

Methods:

Electrochemical analysis and characterization (e.g., cyclic voltammetry, impedance spectroscopy); characterization of enzyme-surface interactions (e.g., atomic force microscopy, fluorescence microscopy); enzyme function assessment on surface and in solution; enzyme engineering (e.g., development of fusion proteins); analysis and characterization of enzyme binding to electrode surfaces; protein biochemistry (expression, purification, stability).

Where to apply:

https://www.circularbioengineering.at/

 

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