Flexibility in Organ Research

In many cases promising results of cell culture experiments lead to a disappointing outcome when transferred to animal experiments or human clinical studies. The reason therefore is obvious. The gap between a two-dimensional cell culture experiment and a complete organism is difficult to bridge, thus results can hardly be related. Aim of this project is the establishment of a 3D Organ Lab Model which helps to investigate the biochemical processes and their pathologies in complete organs. Each organ, based on its function, is composed of specialized cells and has special needs, which are incorporated in this system. This model consists of a scaffold composed of fibers which enable different types of cells the arrangement of a three-dimensional structure similar to the human anatomy, histology, and physiology. These structures can be sectioned in defined regions, where cells are applied to conditions appropriate to their function and requirements (e.g. flow, different nutrition, or oxygen concentration). If needed the 3D Organ Lab Model can be additionally adapted if the cells get in direct cell-to-cell contact or communicate by biochemical factors. Results received from conventional two-dimensional cell culture could be proofed and extended within this system which then will be expanded in animal experiments and clinical studies on patients. Further, animal experiments and time (and money) consuming clinical studies could be reduced by eliminating results of two-dimensional experiments which could not be confirmed in the 3D Organ Lab Model. This project aims in the establishment of a human blood vessel model, a placenta model, and a model of the gut by the use of the 3D Organ Lab Model. Already available systems lack flexibility and do not respond to the needs of specialized cells in a three-dimensional network. This model has following advantages: 1) Different cell types are composed 2) in a three-dimensional network and could be cultivated 3) under diverse physical and chemical conditions 4) in defined regions, wherein 5) direct cell contact can be promoted or avoided. This model consists of an autoclavable und therefore re-usable cartridge and a flexible matrix for one-time use only. Therefore this project addresses companies dealing with manufacturing of cell culture lab ware, but additionally companies developing membranes, fibers, and polymers for medical needs.