Local anesthetics and their possible growth-inhibiting effect on neoplastic central neurones

Previous reports have documented a selective antiprolivative effect of local anesthetics on the growth of neurons in culture. This research project aims to establish the selectivity of this action on neuronal tumour cells and to characterize the mechanism of action. The experiments should also provide further information on the molecular structures, that determine the anti-tumor effect of local anesthetics. It will be necessary to optimize the potency, effectiveness, selectivity and safety. Using electrophysiological methods (voltage sensitive dyes and Ca2+ sensing dyes) and biochemical methods (fluorometric- and ELISA-tests), the physiological properties of these cells under the influence of local anestheties should be further characterized. This may lead to a novel strategy in the treatment of neuronal malignances.