Ex vivo organ-perfusion/conservation of metastastic livers

In vitro and experimental preclinical cancer models in animals are characterized by a number of limitations. In close cooperation with Stefan Schneeberger (Univ. Clinic for Visceral, Transplantation and Thoracic Surgery) and Andreas Pircher (Univ. Clinic for Internal Medicine V), surgically resected parts of the liver with metastases from colorectal cancer will be subjected to extracorporeal normothermic machine perfusion (NMP) to maintain the organ- parts ex vivo. The stability of the novel cancer model will be determined by deep mapping of the tumor and the "healthy" surrounding tissue using high-throughput technologies (scRNASeq, multidimensional FACS, metabolic tracing) during the NMP period. Finally, first pilot experiments of immunological modulation should show the scientific potential of the model.

In vitro and experimental preclinical cancer models in animals are characterized by a number of limitations. In close cooperation with Stefan Schneeberger (Univ. Clinic for Visceral, Transplantation and Thoracic Surgery) and Andreas Pircher (Univ. Clinic for Internal Medicine V), surgically resected parts of the liver with metastases from colorectal cancer will be subjected to extracorporeal normothermic machine perfusion (NMP) to maintain the organ-parts ex vivo. The stability of the novel cancer model will be determined by deep mapping of the tumor and the "healthy" surrounding tissue using high-throughput technologies (scRNASeq, multidimensional FACS, metabolic tracing) during the NMP period. Finally, first pilot experiments of immunological modulation should show the scientific potential of the model.