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Microbial nitrogen cycling: from single cells to ecosystems

Microbial nitrogen cycling: from single cells to ecosystems

Christa Schleper (ORCID: 0000-0002-1918-2735)
  • Grant DOI 10.55776/W1257
  • Funding program Doctoral Programs
  • Status ended
  • Start January 1, 2016
  • End February 28, 2021
  • Funding amount € 2,337,237
  • Project website

Disciplines

Biology (100%)

Keywords

    Nitrogen Cycle, Microbial Communities, Systems Biology, Biofilms, Eukaryote-Microbe Interactions, Microbial Physiology

Abstract

Understanding the contribution of microorganisms to ecosystem processes remains one of the most compelling challenges in ecology and requires a high degree of interdisciplinary research. The Faculty of Life Sciences at the University of Vienna has gathered an exceptional number of renowned experts over the past years with complementary research areas in microbial ecology, functional genomics and aquatic and terrestrial ecosystem research. Eleven faculty members from three departments propose here a joint PhD program with highly integrated, interdisciplinary and international education, training and research, dedicated to creating knowledge and expertise in both microbial ecology and ecosystems research. All proposed PhD projects focus on the nitrogen cycle and its microbial components, a topic to which most members of the faculty have already made significant contributions. We have identified two major themes: (I) Eukaryote - Microbe Interactions and (II) Microbial Metabolic Flexibility and Niche Differentiation, to be tackled in the 22 proposed PhD research topics. All students will enter a program of education and training in basic skills and cutting-edge techniques, including single cell analysis (e.g. Raman and NanoSIMS spectrometry), innovative cultivation strategies, comparative and functional (meta)genomics and - proteomics, as well as community-scale (e.g. Chip-SIP) and ecosystem-scale techniques (e.g. isotope pool dilution techniques, isotope fractionation). The consortium is committed to provide a highly integrated PhD program combining both molecular environmental microbiology and ecosystems research, in particular through (i) the selection of interdisciplinary research projects, (ii) the selection of an interdisciplinary team of (co-)supervisors, (iii) the implementation of individual study plans, (iv) interdisciplinary workshops and lecture series, and (v) secondments to foreign laboratories with complementary expertise. The proposed PhD program will provide an outstanding education, which will shape a new generation of scientists capable of working both conceptually and technically at the interface of molecular microbial ecology and ecosystems research.

Consortium
  • Andreas A. Richter, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Christa Schleper, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Dagmar Wöbken, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Gerhard J. Herndl, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Holger Daims, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Michael Wagner, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Silvia Bulgheresi, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Stefanie Wienkoop, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Thomas Rattei, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
  • Wolfram Weckwerth, Universität Wien
    consortium member (01.01.2016 - 28.02.2021)
Research institution(s)
  • Universität Wien

Research Output

  • 2462 Citations
  • 45 Publications
Publications
  • 2024
    Title Plant roots affect free-living diazotroph communities in temperate grassland soils despite decades of fertilization
    DOI 10.1038/s42003-024-06522-w
    Type Journal Article
    Author Dietrich M
    Journal Communications Biology
    Pages 846
    Link Publication
  • 2018
    Title Candidatus Nitrosocaldus cavascurensis, an Ammonia Oxidizing, Extremely Thermophilic Archaeon with a Highly Mobile Genome
    DOI 10.3389/fmicb.2018.00028
    Type Journal Article
    Author Abby S
    Journal Frontiers in Microbiology
    Pages 28
    Link Publication
  • 2018
    Title Cyanate and urea are substrates for nitrification by Thaumarchaeota in the marine environment
    DOI 10.1038/s41564-018-0316-2
    Type Journal Article
    Author Kitzinger K
    Journal Nature Microbiology
    Pages 234-243
    Link Publication
  • 2018
    Title Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains
    DOI 10.3389/fpls.2018.01558
    Type Journal Article
    Author Jegadeesan S
    Journal Frontiers in Plant Science
    Pages 1558
    Link Publication
  • 2018
    Title Characterization of the First “Candidatus Nitrotoga” Isolate Reveals Metabolic Versatility and Separate Evolution of Widespread Nitrite-Oxidizing Bacteria
    DOI 10.1128/mbio.01186-18
    Type Journal Article
    Author Kitzinger K
    Journal mBio
    Link Publication
  • 2023
    Title Cultivation and genomic characterization of novel and ubiquitous marine nitrite-oxidizing bacteria from the Nitrospirales
    DOI 10.1038/s41396-023-01518-6
    Type Journal Article
    Author Mueller A
    Journal The ISME Journal
    Pages 2123-2133
    Link Publication
  • 2021
    Title Anaerobic Sulfur Oxidation Underlies Adaptation of a Chemosynthetic Symbiont to Oxic-Anoxic Interfaces
    DOI 10.1128/msystems.01186-20
    Type Journal Article
    Author Paredes G
    Journal mSystems
    Link Publication
  • 2020
    Title Composition and activity of nitrifier communities in soil are unresponsive to elevated temperature and CO2, but strongly affected by drought
    DOI 10.1038/s41396-020-00735-7
    Type Journal Article
    Author Séneca J
    Journal The ISME Journal
    Pages 3038-3053
    Link Publication
  • 2020
    Title Genomic and kinetic analysis of novel Nitrospinae enriched by cell sorting
    DOI 10.1038/s41396-020-00809-6
    Type Journal Article
    Author Mueller A
    Journal The ISME Journal
    Pages 732-745
    Link Publication
  • 2019
    Title An automated Raman-based platform for the sorting of live cells by functional properties
    DOI 10.1038/s41564-019-0394-9
    Type Journal Article
    Author Lee K
    Journal Nature Microbiology
    Pages 1035-1048
    Link Publication
  • 2019
    Title Subcellular Phenotyping: Using Proteomics to Quantitatively Link Subcellular Leaf Protein and Organelle Distribution Analyses of Pisum sativum Cultivars
    DOI 10.3389/fpls.2019.00638
    Type Journal Article
    Author Schneider S
    Journal Frontiers in Plant Science
    Pages 638
    Link Publication
  • 2019
    Title Proteomic and Metabolomic Profiling of Deinococcus radiodurans Recovering After Exposure to Simulated Low Earth Orbit Vacuum Conditions
    DOI 10.3389/fmicb.2019.00909
    Type Journal Article
    Author Ott E
    Journal Frontiers in Microbiology
    Pages 909
    Link Publication
  • 2022
    Title Differential regulation of degradation and immune pathways underlies adaptation of the ectosymbiotic nematode Laxus oneistus to oxic-anoxic interfaces
    DOI 10.1038/s41598-022-13235-9
    Type Journal Article
    Author Paredes G
    Journal Scientific Reports
    Pages 9725
    Link Publication
  • 2022
    Title Global Grassland Diazotrophic Communities Are Structured by Combined Abiotic, Biotic, and Spatial Distance Factors but Resilient to Fertilization
    DOI 10.3389/fmicb.2022.821030
    Type Journal Article
    Author Nepel M
    Journal Frontiers in Microbiology
    Pages 821030
    Link Publication
  • 2022
    Title Unexpected Complexity of the Ammonia Monooxygenase in Archaea
    DOI 10.1101/2022.04.06.487334
    Type Preprint
    Author Hodgskiss L
    Pages 2022.04.06.487334
    Link Publication
  • 2019
    Title A Multicolor Fluorescence in situ Hybridization Approach Using an Extended Set of Fluorophores to Visualize Microorganisms
    DOI 10.3389/fmicb.2019.01383
    Type Journal Article
    Author Lukumbuzya M
    Journal Frontiers in Microbiology
    Pages 1383
    Link Publication
  • 2019
    Title Ammonia-oxidizing archaea release a suite of organic compounds potentially fueling prokaryotic heterotrophy in the ocean
    DOI 10.1111/1462-2920.14755
    Type Journal Article
    Author Bayer B
    Journal Environmental Microbiology
    Pages 4062-4075
    Link Publication
  • 2019
    Title Molecular Mechanisms of Tungsten Toxicity Differ for Glycine max Depending on Nitrogen Regime
    DOI 10.3389/fpls.2019.00367
    Type Journal Article
    Author Preiner J
    Journal Frontiers in Plant Science
    Pages 367
    Link Publication
  • 2021
    Title FtsZ-mediated fission of a cuboid bacterial symbiont
    DOI 10.1016/j.isci.2021.103552
    Type Journal Article
    Author Weber P
    Journal iScience
    Pages 103552
    Link Publication
  • 2021
    Title Differential regulation of degradation and immune pathways underlies adaptation of the ectosymbiotic nematode Laxus oneistus to oxic-anoxic interfaces
    DOI 10.1101/2021.11.11.468236
    Type Preprint
    Author Paredes G
    Pages 2021.11.11.468236
    Link Publication
  • 2021
    Title A critical perspective on interpreting amplicon sequencing data in soil ecological research
    DOI 10.1016/j.soilbio.2021.108357
    Type Journal Article
    Author Alteio L
    Journal Soil Biology and Biochemistry
    Pages 108357
    Link Publication
  • 2021
    Title Root exudation of contrasting drought-stressed pearl millet genotypes conveys varying biological nitrification inhibition (BNI) activity
    DOI 10.1007/s00374-021-01578-w
    Type Journal Article
    Author Ghatak A
    Journal Biology and Fertility of Soils
    Pages 291-306
    Link Publication
  • 2021
    Title Increased microbial expression of organic nitrogen cycling genes in long-term warmed grassland soils
    DOI 10.1038/s43705-021-00073-5
    Type Journal Article
    Author Séneca J
    Journal ISME Communications
    Pages 69
    Link Publication
  • 2021
    Title Heat stress response mechanisms in pollen development
    DOI 10.1111/nph.17380
    Type Journal Article
    Author Chaturvedi P
    Journal New Phytologist
    Pages 571-585
    Link Publication
  • 2022
    Title Actin cytoskeleton and complex cell architecture in an Asgard archaeon
    DOI 10.1038/s41586-022-05550-y
    Type Journal Article
    Author Rodrigues-Oliveira T
    Journal Nature
    Pages 332-339
    Link Publication
  • 2017
    Title Cereal Crop Proteomics: Systemic Analysis of Crop Drought Stress Responses Towards Marker-Assisted Selection Breeding
    DOI 10.3389/fpls.2017.00757
    Type Journal Article
    Author Ghatak A
    Journal Frontiers in Plant Science
    Pages 757
    Link Publication
  • 2017
    Title Chemotaxonomic characterisation of the thaumarchaeal lipidome
    DOI 10.1111/1462-2920.13759
    Type Journal Article
    Author Elling F
    Journal Environmental Microbiology
    Pages 2681-2700
    Link Publication
  • 2017
    Title Proteometabolomic response of Deinococcus radiodurans exposed to UVC and vacuum conditions: Initial studies prior to the Tanpopo space mission
    DOI 10.1371/journal.pone.0189381
    Type Journal Article
    Author Ott E
    Journal PLOS ONE
    Link Publication
  • 2017
    Title Candidatus Nitrosocaldus cavascurensis, an ammonia oxidizing, extremely thermophilic archaeon with a highly mobile genome
    DOI 10.1101/240879
    Type Preprint
    Author Abby S
    Pages 240879
    Link Publication
  • 2017
    Title Proteomics survey of Solanaceae family: Current status and challenges ahead
    DOI 10.1016/j.jprot.2017.05.016
    Type Journal Article
    Author Ghatak A
    Journal Journal of Proteomics
    Pages 41-57
  • 2020
    Title Genome wide transcriptomic analysis of the soil ammonia oxidizing archaeon Nitrososphaera viennensis upon exposure to copper limitation
    DOI 10.1038/s41396-020-0715-2
    Type Journal Article
    Author Reyes C
    Journal The ISME Journal
    Pages 2659-2674
    Link Publication
  • 2020
    Title Multiomics approach unravels fertility transition in a pigeonpea line for a two-line hybrid system
    DOI 10.1002/tpg2.20028
    Type Journal Article
    Author Pazhamala L
    Journal The Plant Genome
    Link Publication
  • 2020
    Title Genomic and kinetic analysis of novel Nitrospinae enriched by cell sorting
    DOI 10.1101/2020.06.09.141952
    Type Preprint
    Author Mueller A
    Pages 2020.06.09.141952
    Link Publication
  • 2020
    Title Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales
    DOI 10.3390/biom10111523
    Type Journal Article
    Author Zink I
    Journal Biomolecules
    Pages 1523
    Link Publication
  • 2020
    Title A refined set of rRNA-targeted oligonucleotide probes for in situ detection and quantification of ammonia-oxidizing bacteria
    DOI 10.1016/j.watres.2020.116372
    Type Journal Article
    Author Lukumbuzya M
    Journal Water Research
    Pages 116372
    Link Publication
  • 2020
    Title Flow-through stable isotope probing (Flow-SIP) minimizes cross-feeding in complex microbial communities
    DOI 10.1038/s41396-020-00761-5
    Type Journal Article
    Author Mooshammer M
    Journal The ISME Journal
    Pages 348-353
    Link Publication
  • 2020
    Title The Signal and the Noise: Characteristics of Antisense RNA in Complex Microbial Communities
    DOI 10.1128/msystems.00587-19
    Type Journal Article
    Author Michaelsen T
    Journal mSystems
    Link Publication
  • 2020
    Title Single cell analyses reveal contrasting life strategies of the two main nitrifiers in the ocean
    DOI 10.1038/s41467-020-14542-3
    Type Journal Article
    Author Kitzinger K
    Journal Nature Communications
    Pages 767
    Link Publication
  • 2020
    Title PANOMICS meets germplasm
    DOI 10.1111/pbi.13372
    Type Journal Article
    Author Weckwerth W
    Journal Plant Biotechnology Journal
    Pages 1507-1525
    Link Publication
  • 2020
    Title A refined set of rRNA-targeted oligonucleotide probes for in situ detection and quantification of ammonia-oxidizing bacteria
    DOI 10.1101/2020.05.27.119446
    Type Preprint
    Author Lukumbuzya M
    Pages 2020.05.27.119446
    Link Publication
  • 2018
    Title Metabolomics in Plant Stress Physiology
    DOI 10.1007/10_2017_55
    Type Book Chapter
    Author Ghatak A
    Publisher Springer Nature
    Pages 187-236
  • 2018
    Title A promiscuous beta-glucosidase is involved in benzoxazinoid deglycosylation in Lamium galeobdolon
    DOI 10.1016/j.phytochem.2018.10.012
    Type Journal Article
    Author Hannemann L
    Journal Phytochemistry
    Pages 224-233
    Link Publication
  • 2018
    Title Characterization of a community-acquired-MRSA USA300 isolate from a river sample in Austria and whole genome sequence based comparison to a diverse collection of USA300 isolates
    DOI 10.1038/s41598-018-27781-8
    Type Journal Article
    Author Lepuschitz S
    Journal Scientific Reports
    Pages 9467
    Link Publication
  • 2016
    Title Proteomics and comparative genomics of Nitrososphaera viennensis reveal the core genome and adaptations of archaeal ammonia oxidizers
    DOI 10.1073/pnas.1601212113
    Type Journal Article
    Author Kerou M
    Journal Proceedings of the National Academy of Sciences
    Link Publication
  • 2016
    Title Pollen proteomics: from stress physiology to developmental priming
    DOI 10.1007/s00497-016-0283-9
    Type Journal Article
    Author Chaturvedi P
    Journal Plant Reproduction
    Pages 119-132
    Link Publication

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